Development of a non-invasive diagnostic method for oxygen deficiency based on real-time measurement of methane and nitrous oxide

Goals

  • Developing a non-invasive diagnostic method that enables the early detection of oxygen deficiency or ischemia in the gastrointestinal tract by measuring breath gases (CH4, N2O, and CO2)
  • Scientifically demonstrate that changes in these breath gases are reliably associated with blood flow and microcirculation in the GI tract, as assessed in animal models and human studies
  • Developing a clinically applicable prototype capable of measuring these gases in real time, with the long-term goal of bedside use in clinical practice

Specifically

  • Measuring stable isotope values and concentrations of CH4, N2O and CO2 from collected breath gas samples of human studies and animal models with high accuracy
  • Investigations into NO3- to N2O conversion in mammalian cells

Project Lead

  • Prof. Mihály Boros, Institute for Surgical Research, University of Szeged, Hungary
  • Prof. Frank Keppler & Maurice Maas (PhD Student), Research Group Biogeochemistry, Heidelberg University, Germany
  • Prof. Zoltán Bozóki, Department of Optics and Quantum Electronics, University of Szeged, Hungary
  • Prof. Andrey Kozlov, Ludwig Boltzmann Institute for Traumatology, Vienna, Austria

Cooperation Partners

  • Dr. Reinhard Well & Dr. Caroline Buchen-Tschiskale, Thünen Institute of Climate-Smart Agriculture, Brunswick, Germany

Funding

  • HU-RIZON, International Research Excellence Cooperation Programme of the National Research, Development and Innovation Office, Hungary

Activities & Progress

  • Analysis and evaluation of measurements of the isotopic composition and concentrations of CH4, N2O, and CO2 in breath gas samples from animal models and human studies
•	Measuring stable isotope values and concentrations of CH4, N2O and CO2 from collected breath gas samples of human studies and animal models with high accuracy